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Mosquitoes ignore repellent Deet after first exposure

2013-02-22 13:01:43

By Rebecca Morelle Science reporter, BBC World Service

The widely used insect repellent Deet appears to be losing its effectiveness

against mosquitoes, scientists say.

Researchers from the London School of Hygiene and Tropical Medicine say

mosquitoes are first deterred by the substance, but then later ignore it.

They say more research is needed to find alternatives to Deet, which was first

developed by the US military.

The research was carried out on Aedes aegypti, a species of mosquito that

spreads dengue and yellow fever.

The findings are published in the journal Plos One.

Dr James Logan from the London School of Hygiene and Tropical Medicine, said:

"The more we can understand about how repellents work and how mosquitoes detect

them, the better we can work out ways to get around the problem when they do

become resistant to repellents."

Human bait

Deet - or N,N-diethyl-meta-toluamide - is one of the most widely used active

ingredients in insect repellents. It was developed by the US military,

following its experience of jungle warfare during World War II.

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Mosquitoes are very good at evolving very very quickly

Dr James Logan London School of Hygiene and Tropical Medicine

For many years, it was not clear exactly how the chemical worked, but recent

research suggests that insects simply do not like the smell.

However, there are concerns that some mosquitoes are growing resistant to it.

To find out more, researchers from the London School of Hygiene and Tropical

Medicine took some A. aegypti mosquitoes in the laboratory, and tempted them

with a human arm covered in Deet.

As expected, the repellent put the insects off their potential meal.

However, a few hours later when the same mosquitoes were offered a chance to

dine again, the researchers found that the Deet was less effective.

To investigate why this might be happening, the researchers attached electrodes

to the insects' antenna.

Dr Logan explained: "We were able to record the response of the receptors on

the antenna to Deet, and what we found was the mosquitoes were no longer as

sensitive to the chemical, so they weren't picking it up as well.

"There is something about being exposed to the chemical that first time that

changes their olfactory system - changes their sense of smell - and their

ability to smell Deet, which makes it less effective."

Arms race

Earlier research by the same team found that genetic changes to the same

species of mosquito can make them immune to Deet, although it was not clear if

there were any mosquitoes like this in the wild.

Dr Logan said it was vital to understand both these permanent genetic and

temporary olfactory changes that were taking place.

He said: "Mosquitoes are very good at evolving very very quickly."

He stressed that the findings should not stop people from using Deet in high

risk areas, but that they would help scientists who are trying to find new

versions that could be effective.

To follow up on the study, the researchers now plan to find out how long the

effect lasts after the initial exposure to the chemical.

The team would also like to study the effect in other mosquitoes, including the

species that transmit malaria.