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View submission: Why is bioassay absorbance measured at 2 different wavelengths?
Recently, I did an experiment where we used spectrophotometric analysis results, that was to determine the Lambda max of a particular protein *we used Bovine serum albumin.* At first, we made several different concentrations of that protein and we measured the absorbance of the protein at OD660nm and noted down the results that would help us in plotting the standard curve for determining the Absorbance via calculations. Then we selected a certain concentration of diluted protein and measured its absorbance from 200nm through 400nm and interpreted at what wavelength the absorbance was the highest and hence that was the absorption maxima/lambda max.
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